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# What does Michaelis-Menten plot show?

## What does Michaelis-Menten plot show?

Explanation: In a classic Michaelis-Menten graph, the y-axis represents reaction rate and the x-axis represents substrate concentration. At low substrate concentrations, the reaction rate increases sharply. When a high concentration of substrate is present, all of the enzymes in solution are busy.

What is the units for Vmax?

Vmax “represents the maximum rate achieved by the system, at maximum (saturating) substrate concentrations” (wikipedia). Unit: umol/min (or mol/s).

What is the units for KM?

Kilometre (km), also spelled kilometer, unit of length equal to 1,000 metres and the equivalent of 0.6214 mile (see metric system).

### WHAT IS units in enzyme kinetics?

The enzyme unit, or international unit for enzyme (symbol U, sometimes also IU) is a unit of enzyme’s catalytic activity. 1 U (μmol/min) is defined as the amount of the enzyme that catalyzes the conversion of one micromole of substrate per minute under the specified conditions of the assay method.

Which is Michaelis-Menten equation?

The Michaelis–Menten equation (Eqn (4)) is the rate equation for a one-substrate enzyme-catalyzed reaction. This equation relates the initial reaction rate (v0), the maximum reaction rate (Vmax), and the initial substrate concentration [S] through the Michaelis constant KM—a measure of the substrate-binding affinity.

What is the slope of a Michaelis-Menten plot?

The slope of the line is Km/Vmax, and the y-intercept is 1/Vmax.

#### What is the equation for Vmax?

Vmax is equal to the product of the catalyst rate constant (kcat) and the concentration of the enzyme. The Michaelis-Menten equation can then be rewritten as V= Kcat [Enzyme] [S] / (Km + [S]).

Is km a SI unit?

The kilometre (SI symbol: km; /ˈkɪləmiːtər/ or /kɪˈlɒmɪtər/), spelled kilometer in American English, is a unit of length in the metric system, equal to one thousand metres (kilo- being the SI prefix for 1000)….

kilometre
Symbol km
Conversions
1 km in … … is equal to …
SI units 1000 m

What is the SI unit of length?

metre
The International System of Units (SI): Base units

Base quantity Base unit
length l, x, r, etc. metre
mass m kilogram
electric current I, i ampere
thermodynamic temperature T kelvin

## What is the unit of Vmax and Km?

Vmax is a rate of reaction. It will have units of: or or etc. min sec min Vmax depends on the structure the enzyme itself and the concentration of enzyme present. KM is a the concentration substrate required to approach the maximum reaction velocity – if [S]>>Km then Vo will be close to Vmax.

What are the two meanings of KM in the Michaelis-Menten equation?

Km is the concentration of substrates when the reaction reaches half of Vmax. A small Km indicates high affinity since it means the reaction can reach half of Vmax in a small number of substrate concentration. The double-reciprocal of Michaelis-Menten equation could be used.

What is the formula for the Michaelis-Menten equation?

The model takes the form of an equation describing the rate of enzymatic reactions, by relating reaction rate , the concentration of a substrate S. Its formula is given by This equation is called the Michaelis–Menten equation. Here, represents the maximum rate achieved by the system, happening at saturating substrate concentration.

### How is the Michaelis-Menten kinetics model named?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. It is named after German biochemist Leonor Michaelis and Canadian physician Maud Menten. The model takes the form of an equation describing the rate of enzymatic reactions, by relating reaction rate (rate of formation of product,

How is the Michaelis Menten saturation curve named?

Michaelis–Menten saturation curve for an enzyme reaction showing the relation between the substrate concentration and reaction rate. In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. It is named after German biochemist Leonor Michaelis and Canadian physician Maud Menten.

Which is the maximum reaction velocity in the Michaelis-Menten equation?

At very high substrate concentrations, the rate exhibits saturation, where additional increases in the substrate concentration no longer increase the reaction velocity. This type of saturation kinetics is adequately described by the Michaelis-Menten equation. Vmax is the maximum reaction velocity.