What does Qiagen protease do?
Both QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity and high activity for a wide range of salt, denaturant, detergent, pH, and temperature conditions. Both proteases provide high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality- guaranteed by QIAGEN.
What is the concentration of Qiagen proteinase K?
What is the concentration of the different Proteinase K solutions sold by QIAGEN? Puregene Proteinase K and QIAGEN Proteinase K are both 20 mg/mL.
What is proteinase K used for in DNA extraction?
Proteinase K is used during DNA extraction to digest many contaminating proteins present. It also degrades nucleases that may be present in DNA extraction and protects the nucleic acids from nuclease attack.
How do you store proteinase K?
QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.
What is the difference between protease and proteinase K?
QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues.
What does proteinase K do to the cell?
Proteinase K is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases.
Can you vortex proteinase K?
Always vortex the Proteinase K before use. RNase A (not provided) can be used if RNA-free DNA is required. Use RNase A at the indicated optional step in the protocol.
Does proteinase K digest itself?
Proteinase K is able to digest hair (keratin), hence, the name “Proteinase K”. The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
Is proteinase K toxic?
Proteinase K This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200).
Can you add too much proteinase K?
Proteinase K is a protein and it will constitute a trivial amount of the protein in the extract and will be removed in the Ph-CHCl3 extraction. The SDS might hang around if you use too much, especially if you ethanol precipitate (though I suspect SDS is quite ethanol-soluble).
How does RIPA buffer work?
The following RIPA Lysis Buffer components have the following effects: Tris-HCl is a buffering agent prevents protein denaturation, NaCl is a salt that prevents non-specific protein aggregation, NP-40 is a non-ionic detergent to extract proteins; Na-deoxycholate and SDS are ionic detergents to extract proteins; and …
When to use QIAGEN proteinase K, 2 mL?
Kit contents: Qiagen Proteinase K, 2mL, >600mAU/mL Activity, Ready-to-use Solution, For Protease Digestion During DNA and RNA Preparation, Offer Broad Substrate Specificity with High Activity for a Wide Range of Reaction Conditions, Used in Most DNA and RNA Isolation.
Which is the best adapter for QIAGEN master mix?
Adapter, LightCycler 96 Capillary Rack EQSV0ISAC0 EACH 499 9018958 QIAGEN Master Mix Block, 1x5ml, 4×1.5ml Tubes EQSV0ISAC0
How big is a QIAGEN flip cap adapter?
QIAGEN Adapter, 33×1.8ml screwtop flat baseCryo EQSV0ISAC0 EACH 543 9018950 QIAGEN Flip-Cap Tube Lifter 32×1.5ml Tapered