Useful tips

What is ATCC stands for?

What is ATCC stands for?

The American Type Culture Collection
2The American Type Culture Collection. The American Type Culture Collection (ATCC) is a private, nonprofit organization dedicated to the acquisition, preservation, authentication, and distribution—the “APAD” activities—of diverse biological materials.

What strains ATCC?

ATCC is the premier source for microbial reference strains. These collections include high-quality, fully authenticated certified reference materials, human microbiome specimens, US National Park Service cultures, and eumycetozoan strains. Our rich portfolio of high-quality materials will move your research forward.

Which agents does ATCC currently screen cell lines for?

In addition, ATCC stated “As of January 1, 2010, all human cell lines accessioned into the ATCC general collection are tested for the adventitious agents HIV, Hepatitis B, Hepatitis C, HPV, EBV, and CMV.

What is melanoma cell line?

SK-MEL-3 is one of a series of melanoma cell lines established from patient-derived tumor samples. This cell line is known to form tumors in immunocompromised mice.

What is type strain?

By definition, type strains are descendants of the original isolates used in species and subspecies descriptions, as defined by the Bacteriological Code [14], that exhibit all of the relevant phenotypic and genotypic properties cited in the original published taxonomic circumscriptions.

What are strains in microbiology?

Microbiology and virology A strain is a genetic variant or subtype of a microorganism (e.g., a virus, bacterium or fungus). For example, a “flu strain” is a certain biological form of the influenza or “flu” virus. These flu strains are characterized by their differing isoforms of surface proteins.

What is a type strain?

What are examples of Opim?

Other potentially infectious materials (OPIM) means: (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body …

What are B16 melanoma cells?

B16 melanoma is a murine tumor cell line used for research as a model for human skin cancers. B16 cells are useful models for the study of metastasis and solid tumor formation, and were one of the first effective murine tools for metastasis research.

When to add TPA to a melanoma cell line?

TPA (200nM) was added only after 3 days’ incubation, as preliminary work suggested that TPA inhibits the attachment of epidermal fragments from which many of the melanoblasts emerge. The medium was changed weekly.

What kind of cancer can a Melan A cell line treat?

Melan-a cell line with use to investigate melanoma skin cancer. Skin cancers are skin growths with differing causes and varying degrees of malignancy. The three most common malignant skin cancers are basal cell carcinoma, squamous cell carcinoma, and melanoma.

What are the characteristics of a normal melanocytes cell line?

Cells retain all tested characteristics of normal melanocytes except senescence and a proliferative response to cholera toxin in the presence of tetradecanoyl phorbol acetate (TPA). They have the diploid chromosome number and are non-tumorigenic in syngeneic and nude mice.

Where does the Melan-A cell line come from?

To understand what is abnormal in melanomas, it is clearly desirable to make cellular and molecular comparisons between melanoma cells and the equivalent normal cells, melanocytes. The melanocyte line, melan-a, is derived from the embryonic skin of C57BL mice.