What is c-myc antibody?
The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody.
How does a myc tag work?
How does Myc-tag work? A Myc-tag can be used to detect expression of recombinant proteins. First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples.
What does Myc gene stand for?
GeneCards Summary for MYC Gene MYC (MYC Proto-Oncogene, BHLH Transcription Factor) is a Protein Coding gene. Diseases associated with MYC include Burkitt Lymphoma and High Grade B-Cell Lymphoma With Myc And/ Or Bcl2 And/Or Bcl6 Rearrangement.
What is Myc DDK tag?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope.
What cancers is MYC associated with?
In TCGA datasets, the amplification of Myc occurs in several cancer types, including breast, colorectal, pancreatic, gastric, and uterine cancers. In the experimental transformation process of normal cells into cancer cells, the MYC gene can cooperate with the RAS gene.
What is the difference between MYC and c-Myc?
Myc and c-myc are the same thing, the normal cellular (hence the c) version of this protein, which can be oncogenic when mutated. The myc tag is a 10-residue sequence derived from c-myc, which is a much larger protein.
How big is a Myc tag?
5 x 1 mg
|Size:||5 x 1 mg|
|Research Area / Disease:||Epitope Tag|
|Gene ID Human:||4609|
What is the primary use of his-tags?
The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. These residues readily coordinate with transition metal ions such as Ni2+ or Co2+ immobilized on beads or a resin for purification.
How much antibodies do I put in my IP address?
But a general rule is to add 2 to 10 micrograms of antibody per 500 micrograms of lysate. If you are using neat antisera, or an IgG fraction (such as protein-A purified antibody), greater amounts of antibody are likely to be required.
How are monoclonal antibodies used to detect Myc?
Figure 2A illustrates western blotting of anti-c-Myc monoclonal antibodies to detect both endogenous c-Myc and a c-Myc protein fused to the ligand binding domain of the estrogen receptor (MycER). c-Myc and c-MycER are distinguished by the higher molecular weight of c-MycER fusion protein.
Where is the c-myc tag located in the antibody?
The anti-c-Myc antibody recognizes an epitope located within the amino acid sequence (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-) of the product of the human oncogene c-myc, also known as the c-Myc tag. The antibody detects the c-Myc tag sequence when it is placed at either the amino or the carboxyl terminus of the fusion protein [ 1 ].
What can be used to purify c-Myc tagged proteins?
Monoclonal anti-c- Myc Antibody (Clone 9E10), which recognizes the c-Myc tag (410–419 of the human p62-c-Myc protein), is supplied crosslinked to immobilized Protein A agarose beads. This product may be used to purify or immunoprecipitate c-Myc-tagged fusion proteins from cell lysates.
What is the rating of anti myc antibody 9e10?
c-Myc Antibody (9E10) is rated 4.7 out of 5 by 228 . raised against an epitope corresponding to amino acids 408-439 within the C-terminal domain of c-Myc of human origin