What is the highest DNA band size in 1 kb DNA ladder?
1 kb plus DNA ladders usually have about 14 purified DNA fragments with a reference band at 1000 and 3000 bp for easy orientation. Most 1 kb plus DNA ladders are provided with loading dye which allows a convenient detection of sharp bands after electrophoresis.
How much ladder do I add to gel?
For a standard electrophoresis system, we recommend loading 0.5 µg (20 µl) of the Fast DNA Ladder on the agarose gel. For a fast electrophoresis system (5 to 30 minutes separation), follow the system’s manufacturer recommendations: 5 to 20 µl load.
What is a DNA ladder mix?
Thermo Scientific MassRuler DNA Ladder Mix is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis. The ladders are mixtures of chromatography-purified, individual DNA fragments.
Is DNA ladder double-stranded?
The ladder consists of 13 double-stranded, blunt-end DNA fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1). The 1000 and 3000 bp fragments have greater intensity relative to the other bands for easy identification.
Do DNA ladders expire?
The recommended loading volume of 1 kb DNA Ladder is 6 μL per lane. Storage: Store at -30°C to +30°C. Shelf Life: Shelf Life: Stable until expiry date (EXP) on label. If product is frozen, thaw at room temperature (15 – 25°C).
What is a ladder in gel electrophoresis?
A DNA ladder is a solution of DNA molecules of different lengths used in agarose or acrylamide gel electrophoresis. It is applied as a reference to estimate the size of unknown DNA molecules that were separated based on their mobility in an electrical field through the gel.
Are DNA ladders comprised of single stranded DNA?
Invitrogen DNA ladders are composed of double-stranded DNA fragments only.
What is the purpose of the DNA ladder in gel electrophoresis?
Why do we use a ladder in gel electrophoresis?
What does 1 KB plus DNA ladder do?
Description Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.
How many DNA fragments are in 1 KB plus generuler?
Description Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. Highlights of 1 kb Plus DNA Ladder
When to use generuler 100 BP plus DNA ladder?
Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific GeneRuler 100 bp Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 3,000 bp on agarose or polyacrylamide gels.
How many lanes in quick load plus DNA ladder?
Quick-Load 1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane. Suggested Load: 5 to 10 µl/lane.