What is o-toluidine method?
An o-Toluidine Method for Body-Fluid Glucose Determination o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. The development of the procedure is discussed, as is the specificity of the method for glucose.
What is o-toluidine used for?
ortho-Toluidine is used as an intermediate in the synthesis of the large-volume herbicides, metolachlor and acetochlor, in the manufacture of more than 90 dyes and pigments (e.g. acid-fast dyestuffs, azo pigment dyes, triarylmethane dyes, sulfur dyes, and indigo compounds), and as an intermediate for synthetic rubber …
Is o-toluidine amine?
These isomers are o-toluidine, m-toluidine, and p-toluidine, with the prefixed letter abbreviating, respectively, ortho; meta; and para. All three are aryl amines whose chemical structures are similar to aniline except that a methyl group is substituted onto the benzene ring.
Which functional group of o-toluidine reacts with glucose equation?
The aldehyde group of glucose can undergo condensation with aromatic compounds to yield a colored product. In the most commonly used condensation reaction, o-toluidine reacts with glucose to form a glucosamine that has an intense green color.
What is the principle of O tolidine test?
o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 100° for 10 min., and the absorbance is determined at 630 or 635 mµ.
How do you make Ortho Toluidine reagents?
1) Add 1ml of O-Toluidine reagent to100ml of the sample and mix thoroughly. 2) Allow the sample to stay in dark for 5-15minutes for the colour to develop if the sample is free of chloramine and nitrite and iron are each less than 0.3ppm. If chloramine is present, allow it to stand for full 15 minutes.
Is o-Toluidine soluble in water?
O-toluidine appears as a clear colorless or light yellow liquid. May become reddish brown on exposure to air and light. Flash point 185°F. Has about the same density as water and is very slightly soluble in water.
Is o-Toluidine toxic?
o-Toluidine is primarily used in the manufacture of dyes. o-Toluidine is highly toxic to humans when absorbed through the skin, inhaled as vapor, or swallowed. Acute (short-term) exposure of humans to o- toluidine affects the blood (i.e., methemoglobinemia), with clinical signs of central nervous system depression.
Is O-toluidine soluble in water?
What functional group is p-Toluidine?
methyl group
P-toluidine is an aminotoluene in which the amino substituent is para to the methyl group.
How do you make Ortho Toluidine?
o-Toluidine is produced industrially by nitration of toluene to give mixture of nitrotoluenes, favoring the ortho isomer. This mixture is separated by distillation. 2-Nitrotoluene is hydrogenated to give o-toluidine.
What forms when glucose reacts with O-Toluidine?
Estimation of Blood Glucose by O-toluidine Method16 The protein free filtrate containing glucose reacts with O-toluidine in glacial acetic acid in the presence of heat to form green colored N- glycosylamine.
How is the chemical compound o toluidine produced?
Synthesis and reactions. o-Toluidine is produced industrially by nitration of toluene to give mixture of nitrotoluenes, favoring the ortho isomer. This mixture is separated by distillation. 2-Nitrotoluene is hydrogenated to give o-toluidine.
How is the ortho isomer o toluidine produced?
o -Toluidine is produced industrially by nitration of toluene to give mixture of nitrotoluenes, favoring the ortho isomer. This mixture is separated by distillation. 2-Nitrotoluene is hydrogenated to give o-toluidine.
How to make isatoic anhydride and o toluidine?
Method A A mixture of isatoic anhydride (1.6 g, 0.01 mole) and o-toluidine (1,1 g, 0,01 mol) is heated to 120° for 2 hours. The reaction mixture after cooling is triturated with ether (or dissolve the brown mixture in warm acetone and add water to crash out the crystals).
When did the o toluidine reaction become popular?
The o -toluidine reaction for the estimation of glucose concentration in the serum gained massive popularity in the 1970s. This method was mostly used by clinical laboratories. Because of the potential health hazard, the laboratories now have a modified method by using alternative compounds.