What is a microparticle enzyme immunoassay?
Microparticle enzyme immunoassay. (MEIA) is a technique in which the solid-phase support consists of very small microparticles in liquid suspension. Specific reagent antibodies are covalently bound to the microparticles.
What does enzyme immunoassay detect?
Enzyme immunoassays (EIA) are used to visualize and quantify antigens. They use an antibody conjugated to an enzyme to bind the antigen, and the enzyme converts a substrate into an observable end product. The substrate may be either a chromogen or a fluorogen.
What enzyme is highly used in ELISA?
The most commonly used enzyme labels are horseradish peroxidase (HRP) and alkaline phosphatase (AP). Other enzymes have been used as well; these include β-galactosidase, acetylcholinesterase, and catalase. A large selection of substrates is available commercially for performing ELISA with an HRP or AP conjugate.
How does the direct Elisa work?
In a direct ELISA, an antigen or sample is immobilized directly on the plate and a conjugated detection antibody binds to the target protein. Substrate is then added, producing a signal that is proportional to the amount of analyte in the sample.
What is the use of enzyme immunoassay?
Enzyme immunoassay (EIA) is now widely used as a diagnostic tool in various industries as well as medicine. They can also be used as analytical tools for detecting particular antigens or antibodies in a certain sample during biomedical research.
What are the types of enzyme immunoassay?
Five types of immunoassay, enzyme immunoassay (EIA), radioimmunoassay (RIA), fluoroimmunoassay (FIA), chemiluminescent immunoassay (CLIA) and counting immunoassay (CIA), are generally used.
What is the purpose of fluorescence polarization?
Fluorescence polarization (FP) is a homogeneous method that allows rapid and quantitative analysis of diverse molecular interactions and enzyme activities.
Why Fluorescence Polarization Immunoassay is important?
Fluorescence polarization immunoassay (FPA) is a homogeneous immunoassay useful for rapid and accurate detection of antibody or antigen. Because it is a primary antigen-antibody interaction, the rate of reaction is very rapid and usually a result may be obtained in minutes.
What are the four types of ELISA?
Choosing the right type of ELISA There are four main types of ELISA: direct ELISA, indirect ELISA, sandwich ELISA and competitive ELISA. Each has unique advantages, disadvantages and suitability.
What is the emit 2000 tacrolimus assay used for?
Monitoring tacrolimus levels is essential to ensuring efficacy of treatment while minimizing toxic side effects and preventing organ rejection. The EMIT ® 2000 tacrolimus method is a convenient, easy to use solution for routine tacrolimus monitoring. No significant hematocrit interference 1,2
What are the pharmacokinetics of tacrolimus in humans?
Tacrolimus shows considerable variability in its pharmacokinetics, with poor correlation between the tacrolimus trough level and systemic exposure, as measured by the AUC of concentration time.
How is tacrolimus measured in an organ transplant program?
Methods of Analysis Monitoring of tacrolimus is an integral part of any organ transplant program because of variable dose-to-blood concentrations and the narrow therapeutic index. Tacrolimus can be measured using enzyme-linked immunosorbent assay (ELISA), semi-automated and automated immunoassay, and HPLC-MS (Table 6).
Are there any immunoassays that cross react with tacrolimus?
All the immunoassays have significant cross-reactivities with tacrolimus metabolites. The ELISA, MEIA II, and EMIT cross-react with M-II (31-o-demethyl), M-III (15-o-demethyl) and M-V (15,13-di-o-demethyl) metabolites of tacrolimus (112).